Each student (not each pair) should make the 1/37 dilution four times, identically, to test reproducibility.Record the serial numbers of the pipeters you use (if they have serial numbers) else label the pipeters with tape and marker.What do you need to know in order to make a dilution which will meet the needs of the situation?) (Suppose you are in a research lab and you need to make a dilution of a concentrated reagent stored in the refrigerator. What do you need to know? Before making your dilution, make a list of things you need to know, and check it with your instructors.Make a dilution which reduces the concentration by 37-fold, which is a 1/37 dilution. You are provided with a concentrated stock solution of a yellow solute (p-nitrophenol), labeled 'NP Stock'. It is important to know how to design and perform dilutions which are accurate and which meet the needs of the situation. Reagent dilutions are needed daily in biochemical or immunological lab work. Verify instrument performance with simple tests.Download the sims 3 pc tasik game minecraft. Pros and cons of conventional spectrophotometer vs.ĩ6-well plate absorbance reader.Determine protein concentration with spectrophotometer.Determine precision and accuracy of dilutions.Understand how to calculate optimal dilutions.Spectrophotometry & Dilutions Microbiology 542 - Eric Martz Goals: 1 ml added to 9 ml gives a 10-fold dilution 1 ml added to 99ml. Serial dilution involves repeatedly mixing known amounts of source culture with (sterilised) liquid.
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